Journal: The Journal of Neuroscience

Article Title: Tonic and Phasic Nitric Oxide Signals in Hippocampal Long-Term Potentiation

doi: 10.1523/JNEUROSCI.2259-06.2006

Figure Lengend Snippet: Identification of selective nNOS inhibitors. A, Concentration-response curves for NMDA and DEA/NO (both 2 min exposure) on cGMP levels in rat hippocampal slices incubated with the phosphodiesterase inhibitor EHNA (300 μm). B, Concentration-dependent inhibition of the cGMP response to 100 μm NMDA (in the presence of EHNA) by 1400W and l-VNIO. C, Summary of the effects of 1400W (1 μm), NPA (1 μm), and l-VNIO (0.1 μm) on NMDA-stimulated cGMP levels. D, Lack of effect of these nNOS/iNOS inhibitors on acetylcholine (ACh)-induced cGMP elevation in rings of rat aorta, a response inhibited by the nonselective NO synthase inhibitor l-NNA (100 μm). The exposure to acetylcholine was for 1 min, and the experiments in C and D were performed in parallel using the same reagents. *p < 0.001 compared with NMDA or acetylcholine alone (control 2); n = 5 (all panels). Data were analyzed by one-way ANOVA and Tukey's post hoc test.

Article Snippet: Membranes were incubated overnight at 4°C with monoclonal antibodies against eNOS, iNOS, and nNOS (BD Biosciences, Oxford, UK; all 1:2000).

Techniques: Concentration Assay, Incubation, Inhibition

Journal: The Journal of Neuroscience

Article Title: Tonic and Phasic Nitric Oxide Signals in Hippocampal Long-Term Potentiation

doi: 10.1523/JNEUROSCI.2259-06.2006

Figure Lengend Snippet: Origin of tonic NO in rat hippocampal slices. The nNOS/iNOS inhibitors 1400W (1 μm), NPA (1 μm), and l-VNIO (0.1 μm) failed to reduce cGMP levels in the presence of BAY 41–2272 (p = 0.66–1), whereas the nonselective NO synthase inhibitors l-NIO (100 μm) and l-NNA (100 μm) were both effective (*p < 0.001). The NMDA antagonist d-AP5 (100 μm), AMPA antagonist CNQX (100 μm), and Na + channel blocker tetrodotoxin (TTX, 1 μm) did not change cGMP levels in the presence of BAY 41–2272 significantly (p = 1), whereas ODQ, which blocks NO-stimulated GC activity, was strongly inhibitory. *p < 0.001; n = 8–14. Data were analyzed by one-way ANOVA and Tukey's post hoc test.

Article Snippet: Membranes were incubated overnight at 4°C with monoclonal antibodies against eNOS, iNOS, and nNOS (BD Biosciences, Oxford, UK; all 1:2000).

Techniques: Activity Assay

Journal: The Journal of Neuroscience

Article Title: Tonic and Phasic Nitric Oxide Signals in Hippocampal Long-Term Potentiation

doi: 10.1523/JNEUROSCI.2259-06.2006

Figure Lengend Snippet: Tonic NO in wild-type and eNOS knock-out hippocampal slices. A, BAY 41–2272-stimulated cGMP accumulation in wild-type slices was unaffected (p = 0.97–0.98) by the nNOS/iNOS inhibitors 1400W (1 μm), NPA (1 μm), and l-VNIO (0.1 μm) or by 100 μm d-AP5 (p = 0.38) but was significantly reduced by l-NIO and l-NNA (both 100 μm), which are nonselective NO synthase inhibitors, or by ODQ (10 μm), an inhibitor of NO-activated guanylyl cyclase (*p < 0.001). B, At the same concentrations used in A, none of the NO synthase inhibitors significantly reduced BAY 41–2272-stimulated cGMP accumulation in slices from eNOS knock-out mice (p = 0.2–1). d-AP5 was also ineffective (p = 0.8), but the inhibition by ODQ was just significant. *p = 0.04; n = 15 in all cases. Data were analyzed by one-way ANOVA and Tukey's post hoc test.

Article Snippet: Membranes were incubated overnight at 4°C with monoclonal antibodies against eNOS, iNOS, and nNOS (BD Biosciences, Oxford, UK; all 1:2000).

Techniques: Knock-Out, Inhibition

Journal: Cell

Article Title: A Macrophage Response to Mycobacterium leprae Phenolic Glycolipid Initiates Nerve Damage in Leprosy

doi: 10.1016/j.cell.2017.07.030

Figure Lengend Snippet:

Article Snippet: Anti-iNOS (clone 54) , BD Biosciences , Cat#610431.

Techniques: Transformation Assay, Expressing, Recombinant, Isolation, Derivative Assay, Sequencing, Labeling, Plasmid Preparation, Software, Fluorescence, Imaging